The Effect of Genetic Polymorphism of rs2283468 of IL-32 Gene in Patients with Echinococcus granulosus.

Abstract

The E. granulosus is recognized as a biologically distinct subspecific variant or strain that may exhibit varying degrees of infectivity toward domestic animals and humans. This research examined the influence of the IL-32 gene polymorphism rs2283468 in patients with Echinococcus granulosus. This study has been ongoing from January 2024 until the end of May. Five ml of blood samples were collected from thirty subjects, including fifteen patients with cystic echinococcosis from the hospitals in Baghdad city and fifteen healthy persons as a control group, and fifteen hydatid cyst samples were collected from infected humans that had undergone a surgical operation. Conventional PCR with specific primers, followed by Sanger sequencing, was used to characterize the SNP. We analyzed the gene using Sanger sequencing for the SNP rs2283468 of IL-32, and found that patients with Echinococcus granulosus had genotype frequencies of AA (13.3%), CC (73.3%), and AC (13.4%). Also, in healthy people, there was AA (80.0%), CC (0.0%), and AC (20.0%). The CC genotype got the highest score in patients (73.3%) and the lowest score in healthy people (0.0%). This was in contrast to the dominant allele, which got the highest score in healthy people (80%) and the lowest score in patients (13.3%), with very different (p<0.05) and odd ratios (0.008). The C allele is more common in patients (75.0%) and least common in healthy people (16.67%), with a significant difference (p< 0.05).